다양한 식품에서 Campylobacter jejuni 검출을 위한 real-time PCR과 배지배양법의 비교검증
Comparison of Real-Time PCR and Culture Methods for Detection of Campylobacter jejuni in Various Foods
In this study, performances of culture methods using two selective media and real-time PCR were evaluated for detection of Campylobacter jejuni (C. jejuni) in various food samples. Sausage, ground beef, and radish sprouts inoculated with C. jejuni were enriched in Hunt broth and then streaked onto modified cefoperazone charcoal deoxycholate agar and Preston agar, followed by incubation under microaerobic conditions. The enriched Hunt broth (1 mL) was used in real-time PCR assay. No statistical differences were observed in sensitivity among the two selective media and real-time PCR for sausage and ground beef. However, the number of positives by real-time PCR in radish sprouts was much higher than the two selective media (p<0.05). It appears that real-time PCR could be used as an effective screening tool to detect C. jejuni, particularly in foods with a high number of background microflora such as fresh vegetables.
목차
Abstract 서론 재료 및 방법 실험에 사용된 균주 식품샘플의 준비와 접종 배지배양법을 이용한 C. jejuni의 검출 DNA 추출과 Real-time PCR의 실시 식품 내 정상세균총의 측정 자료의 분석 결과 및 고찰 식품샘플 내 정상세균총의 수준과 영향 Real-time PCR과 배지배양법의 검출능력비교 요약 감사의 글 문헌
키워드
Campylobacter jejunireal-time PCRculture methodselective media
저자
천정환 [ Jung-Whan Chon | 건국대학교 수의과대학 ]
현지연 [ Ji-Yeon Hyeon | 건국대학교 수의과대학 ]
황인균 [ In-Gyun Hwang | 식품의약품안전청 식품미생물팀 ]
곽효선 [ Hyo-Sun Kwak | 식품의약품안전청 식품미생물팀 ]
한정아 [ Jeong-A Han | 식품의약품안전청 식품미생물팀 ]
김무상 [ Moo-Sang Kim | 서울시 보건환경연구원 역학조사팀 ]
김종현 [ Jong-Hyun Kim | 질병관리본부 장내세균과 ]
송광영 [ Kwang-Young Song | 건국대학교 수의과대학 ]
서건호 [ Kun-Ho Seo | 건국대학교 수의과대학 ]
Corresponding author