Earticle

초록

영어

Chinese fir (Latin name: Cunninghaimia lanceolata) is one of the major commercial coniferous trees. Most of Chinese fir forests are managed in successive rotation sites, which lead productivity to decline. Autotoxicity is the important reason for soil degradation of Chinese fir plantation, especially, phenolic acids are considered as the major allelopathic toxins which
induce autotoxicity in Chinese fir rotation stands. We performed here proteomic approach to investigate the response of proteins in Chinese fir leaves to salicylic acid. The tube plantlets of Chinese fir clone were treated with 120 mg/L salicylic acid for 1, 3 and 5th day. 2-DE, coupled with MALDI-TOF-TOF/ MS, was used to separate and identify the responsive proteins. We found
12, 7, and 12 candidate protein spots that were up- or down-regulated by at least 2.5 fold after 1, 3, and 5th day of the stress, respectively. Of these protein spots, 16 spots were identified successfully. According to the putative physiological functions, these proteins were categorized into five classes (1) the proteins involved in protein stability and folding, including 26S proteome, Grp78, Hsp70, Hsp90 and PPIase; (2) the protein involved in photosynthesis and respiration, including OEC 33 kDa subunit, GAPDH; (3) the protein related to cell endurance to acid, F-ATPase; (4) the protein related to cytoskeleton, tubulin; (5) the protein related to protein translation: prolyl-tRNA synthetase. These results give new insights into autotoxic substance stress response in Chinese fir leaves and provide preliminary footprints for further studies on the molecular signal mechanisms induced by the stress.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Plant Materials and Salicylic Acid Treatement
  Protein Extraction
  Two-Dimensional Gel Electrophoresis
  Gel Imaging and Data Analysis
  In-Gel Tryptic Digestion
  Identification of Proteins by Mass Spectrometry
 RESULTS AND ANALYSIS
  2-DE Analysis of Leave Proteins in Chinese Fir Treated by Salicylic Acid
  The Identification of Responsive Proteins to Salicylic Acid by MS/MS
 DISCUSSION
  OEC 33 kDa Subunit
  26S Proteasome
  Peptidyl-Prolyl Cis-Trans Isomerase
  F0F1-ATPase
  Glyceraldehyde-3-Phosphate Dehydrogenase
  Tubulin
  Amino acyl-tRNA Synthetase
 CONCLUSION
 Literature Cited

키워드

저자

• Mei Yang [ College of Forestry, Guangxi University, 530004, Nanning, China ] Corresponding author
• Sizu Lin [ College of Forestry, Fujian Agriculture and Forestry University, 350002, Fuzhou, China ]
• Guangqiu Cao [ College of Forestry, Fujian Agriculture and Forestry University, 350002, Fuzhou, China ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Journal of Forest and Environmental Science [산림과학연구]

• 간기

  계간

• pISSN

  2288-9744

• eISSN

  2288-9752

• 수록기간

  1981~2025

• 등재여부

  KCI 등재

• 십진분류

  KDC 526 DDC 634