The purification of protein has been widely used in the electrodialysis, ultrafiltration, microfiltration when comparing with the number of studies on the use of conventional membrane for different proteins’ separation [1~2]. But they were not used as a purpose of enhancing the permeability. The newly developed techniques superimpose additional forces such as the pressured hydraulic force. However, the principle disadvantage of this technique is that cannot be separated in high concentration of protein. In this paper a superimposed another force, that is, an electric field induces as a force on charged protein in order to reduce the surface layer on the membrane. A newly developed type by an electric force has been invented a electro-microfiltration membrane [3~5]. Present studies in this article are experimentally to test the performance of new-typed electro-microfiltration membrane at a low hydraulic pressure and induces high amount of permeates. As such, the membrane filtration was investigated based on the membrane properties measured experimentally using the cake filtration theory [6]. The microfiltration technique was chosen to reduce the transmembrane pressure (TMP) and to increase the permeate flux(see below figures). This study shows that this membrane system manufactured by a electro-microfiltration technique offers significant advantages. The purification of protein in this presented work is to investigate in the membrane process under the influence of an electric field. This paper presents with example from membrane process showing how the filtration time is reduced by the use of an electric field. The transmembrane pressure (TMP) was reduced by 20 % as the electric field increased. The concentration of protein in the membrane process in the presence of an electric field was reduced by over 300 % in comparison with the membrane process without an electric field. The hydraulic electrofiltration provides an another substitute to the crossflow filtration in the purification of protein.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동