Earticle

초록

영어

Cardiovascular disease is generally caused by a blockage of blood flow through heart or brain. The most common cause for blockage is a build-up of fatty deposits, inflammation and atherosclerotic plaques on the inner walls of blood vessels. It is assumed that cardiovascular disease related proteins would be secreted from the deposits or plaque on the inner wall of blood vessels. If it is possible to monitor the increase or decrease of these proteins, the incident of cardiovascular disease can be prevented. In this study, to identify the changed proteins for diagnosis of cardiovascular disease, protein profiles of human sera 20 of non-diabetic cardiovascular disease patients were compared with those of 30 healthy controls using the two-dimensional gel electrophoresis technique. Before 2-DE, high abundant proteins in serum were removed using the multiple affinity removal column connected with HPLC system. The differentially expressed spots in cardiovascular disease patients were identified by ESI-Q-TOF mass spectrometry. The identified proteins were confirmed via Western blot analysis. Among the identified proteins, RBP4 and Celluloplasmin were confirmed to be good candidates for diagnostic and monitoring biomarkers of cardiovascular disease.

키워드

저자

• Won-Young JEONG [ School of Life Sciences and Biotechnology, Korea University, Seoul, Korea. ]
• Hyun-Syuk YOO [ School of Life Sciences and Biotechnology, Korea University, Seoul, Korea. ]
• Hyun-Jung KIM [ School of Life Sciences and Biotechnology, Korea University, Seoul, Korea. ]
• Pan-Kyeom KIM [ School of Life Sciences and Biotechnology, Korea University, Seoul, Korea. ]
• Mi-Ryung KIM [ School of Life Sciences and Biotechnology, Korea University, Seoul, Korea. ]
• Chan-Wha KIM [ School of Life Sciences and Biotechnology, Korea University, Seoul, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576