Earticle

초록

영어

We have developed a new strategy in which a polymerase enzyme is controlled to accomplish an unnatural extension reaction even at the mismatched site of a primer with template DNA. The validity of this novel concept was systematically demonstrated by using Hg2+ and Ag+ ions to intentionally trigger an unusual illusionary polymerase activity at respective T-T and C-C mismatched primers on the basis of their specific interactions with the respective mismatched base pairs T-T and C-C. By utilizing this new concept, a molecular scale logic gate system has been successfully constructed by rationally designing primers and selecting the type of DNA polymerase employed. The most notable feature of the logic gates devised herein is their simplicity and cost-effective design since the only requirement for construction of the new logic gates is the incorporation of a single mismatched base (T and C) at the 3’ end of the primer and the application of metal ions (Hg2+ and Ag+). Moreover, this novel concept that the unnatural polymerase activity could be triggered by metal ions could be utilized to develop new strategies for the detection of metal ions (Hg2+ and Ag+).

키워드

저자

• Ki Soo PARK [ Dept. of chemical & Biomolecular engineering, KAIST, Daejeon, 305-701. ]
• CheulHee JUNG [ Dept. of chemical & Biomolecular engineering, KAIST, Daejeon, 305-701. ]
• Hyun Gyu PARK [ Dept. of chemical & Biomolecular engineering, KAIST, Daejeon, 305-701. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576