Various methods have been developed to concentrate bacteria from the raw sample by their coagulations and sedimentations. However, these methods did not prefer to low concentration bacteria in the real world applications. In this study, we developed simple concentration method of gram negative and positive bacteria (Escherichia coli and Bacillus cereus, respectively). High concentrated bacteria (gram negative and positive) were well isolated using hydrophilic surface under salty condition (pH4, 100mM of phosphate) whereas the adsorption efficiencies of bacteria were dramatically decreased in case of low concentrated bacteria. We optimized the bacteria adsorption on hydrophilic surface using aquatic chemistry. The optimal conditions were determined with PEG and divalent cations for E. coli and with divalent anions for B. cereus. The adsorption rate increased up to 50% when low concentration bacteria less than 102c.f.u were applied with optimal conditions. As a result, this method could be directly applied to detect E. coli in low concentration in beef sample [101c.f.u/g]. Acknowledgment: This subject is supported by Ministry of Environment of the Republic of Korea as “The Eco-Technopia 21 project” and by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MEST) (No.M10755160002-20100020657))
키워드
hydrophilic surfacePEGdivalent ion
저자
Ji Yeong WON [ Department of Chemical and Biomolecular Engineering, Sogang University, #1 Shinsu-dong, Mapo-gu, Seoul 121-742, Republic of Korea. ]
JunHong MIN [ College of Bionano technology, Kyungwon University, Bokjung-dong, Sujung-gu, Sungnam-si, Gyunggi-do 461-701, Republic of Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
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