Earticle

초록

영어

A lipid bilayer plays an important role in studying ion channels.
However, a lipid bilayer created using conventional techniques is not storable and automatable, precluding practical applications. We have formerly described a method of forming a droplet interface bilayer by falling an aqueous droplet on the interface created between aqueous and organic phase. Here we demonstrate a storable lipid bilayer precursor frozen with ion channels implementing DIB. A glass vial with aqueous solution and organic solution, 4:1 mixture of hexadecane and n-decane with 3% asolectin dissolved, was flash frozen in -80℃ methanol immediately when an aqueous droplet was released. Upon thawing, the gravity automatically draws the droplet down to the interface along the electrode to initiate lipid bilayer formation. Lipid bilayer formed and incorporation of ion channel alpha-hemolysin showed typical capacitance and conductance consistent with previous literatures. This golden method as a whole can be automated, leading to fully labor free task that can be carried out in any place, increasing the accessibility of ion channel studies and applications for nanobio sensors, drug screening and DNA sequencing.

키워드

저자

• Joong-Jin PARK [ Dept. of Biological engineering, Inha University, Incheon, 402-751. ]
• Sang-Baek CHOI [ Dept. of Biological engineering, Inha University, Incheon, 402-751. ]
• Tae-Joon JEON [ Dept. of Biological engineering, Inha University, Incheon, 402-751. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576