Neurotransmitters has been implicated in a number of physiological and behavioral functions, such as control and regulation of affective behavior, sleep, vigilance, reward system, motor and cognitive function, and neuroendocrine function. The nanotechnology-based bio-barcode- amplification analysis offers an innovative approach to detect dopamine. We evaluated the efficacy of this protein-detection method in detecting dopamine in normal and oxidative stress damaged dopaminergic cells. In this study, a coupling of DNA barcode and bead-based immnunoassay for detecting neurotransmitter with PCR-like sensitivity is reported. The method relies on magnetic nanoparticles with antibodies and nanoparticles that are encoded with DNA and antibodies that can sandwich the target protein captured by the nanoparticle-bound antibodies. The aggregate sandwich structures are magnetically separated from solution, and treated to remove the conjugated barcode DNA. The DNA barcodes were identified by PCR analysis. The concentration of dopamine in dopaminergic cell can be easily and rapidly detected using bio-barcode assay. The bio-barcode assay is a rapid and high-throughput screening tool to detect of neurotransmitter such as dopamine. Acknowledgement: The research was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (2009-0080860) and by the Nano/Bio Science &Technology Program (M10536090001-05N3609-00110) of the Ministry of Science and Technology (MOST) and by Ministry of Environment of the Republic of Korea as "The Eco-technopia 21 project".
키워드
NeurotransmittersgoldnanoparticlesBarcode assay
저자
Jeung Hee AN [ Department of Chemical & Biomolecular Engineering, Sogang University, Seoul121-742. ]
Byung-Keun OH [ Department of Chemical & Biomolecular Engineering, Sogang University, Seoul121-742. ]
Jeong Woo CHOI [ Department of Chemical & Biomolecular Engineering, Sogang University, Seoul121-742. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
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