Earticle

초록

영어

The autodisplay technology is one of the surface display methods of proteins or peptides to the E.coli outer membrane. In the autodisplay techonolgy, proteins or peptides are expressed as a recombinant protein in the autotransporter domain of a protein called AIDA-I (adhesin involved in diffuse adherence) from E.coli. In this work, the autodisplay of streptavidin, a protein with biotin-binding activity, is presented. The autodisplay of streptavidin was confirmed by SDS-PAGE of the outer membrane proteins, and the number of autodisplayed streptavidin molecules on a single E.coli cell was estimated by using densitometric analysis. The biotin-binding activity of the autodisplayed streptavidin was estimated after treating fluorescence labeled biotins by using fluorescence microscope and FACS. The biotin-binding activity of the E.coli with autodisplayed streptavidins was compared with similar sized magnetic beads with covalently immobilized streptavidins.
Finally, the outer membrane with autodisplayed streptavidin was isolated and layered on a 96-well microplate and the biotin-binding activity was estimated to determine whether it is applicapable to immunoassays.

키워드

저자

• Min PARK [ Department of materials science and engineering. ]
• Jo Il KIM [ Department of materials science and engineering. ]
• Ju Kyung LEE [ Department of materials science and engineering. ]
• Jae Chul PYUN [ Department of materials science and engineering. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576