A new covalent immobilization method for small proteins and short peptides is presented by using parylene-H film which, is a polymer of p-xylene having formyl groups. The covalent coupling of proteins to the parylene-H film is produced by only one step of incubation of proteins or peptides without additional coupling reagents. In this work, the parylene-H film is coated on a 96-well microplate for immunoassays. The immobilization efficiency to the parylene-H film was compared with the conventional physical adsorption by using human chorionic gonadotrophin protein and a small peptide called circulated citrullinated peptide as model molecules. Additionally, the applicability of this immobilization method for short peptides is demonstrated by detecting autoantibodies in rheumatoid arthritis patient serum.
키워드
Paryleneimmunoassaycovalent immobilization
저자
Hyuk KO [ Department of materials science and engineering. ]
Jo Il KIM [ Department of materials science and engineering. ]
Eun Hyeng LEE [ Department of materials science and engineering. ]
Jae Chul PYUN [ Department of materials science and engineering. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동