Earticle

초록

영어

Transgenic plant cell cultures are an attractive expression system for the production of industrial and pharmaceutical proteins because of their advantages in safety and low production cost. Human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) was produced and secreted when sugar was depleted in culture medium by transgenic plant cell lines (Oryza sativa L.) using RAmy3D promoter. Formation of reactive oxygen species (ROS) is an inevitable consequence of the aerobic organisms such as animal and plant cells. ROS gives rise to biotic and abiotic stresses. Therefore, ROS levels need to be controlled and the addition of antioxidants can reduce apoptosis caused by ROS which generated during cell cultures with aeration. Many researchers reported the effects of antioxidants, such as glutathion, ascorbic acid (vitamin C), and vitamin E on plant cells. In this study, we investigated the effects of N-acetylcystein, ascorbic acid and reduced glutathion on cell growth and protein production in transgenic rice cell cultures under oxidative stress. Effects of antioxidants with various concentrations were studied by comparing protein production levels using ROS markers. In conclusion, long-term survival of transgenic rice cells and with enhanced hCTLA4Ig production were possible by reducing the level of ROS with the additions of various antioxidants in transgenic rice cell cultures.

키워드

저자

• Hyung-Jin NAM [ Dept. of biological engineering, INHA University, Incheon, 402-751. ]
• Jun-Young KWON [ Dept. of biological engineering, INHA University, Incheon, 402-751. ]
• Su-Hwan CHEON [ Dept. of biological engineering, INHA University, Incheon, 402-751. ]
• Yong-Suk YANG [ Dept. of biological engineering, INHA University, Incheon, 402-751. ]
• Mi-Hee YOO [ Dept. of biological engineering, INHA University, Incheon, 402-751. ]
• Ji-Won CHOI [ Dept. of biological engineering, INHA University, Incheon, 402-751. ]
• Soo-Jin KIM [ Dept. of biological engineering, INHA University, Incheon, 402-751. ]
• Dong-Il KIM [ Dept. of biological engineering, INHA University, Incheon, 402-751. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576