Earticle

초록

영어

Insect cell-baculovirus vector system is one of the promising method for the production of recombinant proteins. Baculovirus produces heterologous recombinant proteins under polyhedrin promoter and is safe because it has a limited host range. Insect cells are easy to cultivate and have post-translational modifications. The development of Porcine Cholera Marker Vaccine is urgently required, because economical losses are very big on pig breeding market in Korea as well as around world. So insect cell-baculovirus vector system was used in this study for production of Porcine Cholera Marker Vaccine. Since Serum has been commonly used in insect cells culture, 10% TNM-FH medium was used for the production of recombinant Porcine Cholera Marker Vaccine by using several insect cell lines of Sf9, Sf21, and Hi-Five. The Sf9 cell growth was highest when insect cells were inoculated at 1x106 cells/mL and 5x105 cells/mL. The effects of multiplicity of infection and harvest time on marker vaccine production were investigated in each case of individual cell lines. As a result, the highest productivity of Porcine Cholera Marker Vaccine achieved was 22.5μg/mL in the condition of 0.001 MOI and 5 days of harvest time with Sf9 cells.
However, the highest virus titer obtained was 7.25x106 pfu/mL at the condition of 10 MOI and 3 days of harvest time with SF9 cells.

키워드

저자

• Je-Sin WOO [ Department of Medical Biotechnology. Kangwon National University, Chunchon, 200-701. ]
• Chang-Jin LEE [ Department of Medical Biotechnology. Kangwon National University, Chunchon, 200-701. ]
• Jung-Min CHOI [ Department of Medical Biotechnology. Kangwon National University, Chunchon, 200-701. ]
• Yeon-Ho JEONG [ Department of Medical Biotechnology. Kangwon National University, Chunchon, 200-701. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576