Earticle

초록

영어

Human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) was produced inducibly by sugar starvation using RAmy3D promoter in transgenic rice cell suspension cultures. Although RAmy3D promoter is known to be the strongest promoter in plant systems, main obstacle is that the target protein was produced in sugar-free conditions. To improve the production of hCTLA4Ig, we applied anoxia conditions with 30 mM of glucose to the production phases.
The anoxia conditions with 30 mM of glucose at the production phases markedly improved cell viability under anoxia conditions. A viability level of more than 65% could be maintained for up to 30 days. Furthermore, the repression of the RAmy3D promoter by residual sugar in the production of hCTLA4Ig was not observed under anoxia conditions with 30 mM of glucose.
In addition, the production periods of hCTLA4Ig was expanded up to 30 days, and the maximum production levels of hCTLA4Ig were 29 mg/L under anoxia conditions and 14 mg/L in aerobic conditions, respectively. Under anoxia conditions with 30 mM of glucose, alcohol dehydrogenase II gene was found to be strongly expressed. Therefore, we suggest that anoxia conditions could be used to achieve energy source by ethanolic fermentation for the improvement of the productivity of hCTLA4Ig in transgenic rice suspension cell cultures.

키워드

저자

• Jun-Young KWON [ Department of Biological Engineering, Inha University, Incheon 402-751, Korea. ]
• Su-Hwan CHEON [ Department of Biological Engineering, Inha University, Incheon 402-751, Korea. ]
• Ji-Won CHOI [ Department of Biological Engineering, Inha University, Incheon 402-751, Korea. ]
• Yong-Suk YANG [ Department of Biological Engineering, Inha University, Incheon 402-751, Korea. ]
• Mi-Hee YOO [ Department of Biological Engineering, Inha University, Incheon 402-751, Korea. ]
• Soo-Jin KIM [ Department of Biological Engineering, Inha University, Incheon 402-751, Korea. ]
• Hyung-Jin NAM [ Department of Biological Engineering, Inha University, Incheon 402-751, Korea. ]
• Dong-Il KIM [ Department of Biological Engineering, Inha University, Incheon 402-751, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576