Earticle

초록

영어

Various challenges have been attempted to increase the production of therapeutic proteins in mammalian cell culture. Process parameters such as pH, temperature and osmolarity have been shown to exert a significant effect on cell line performance. In this study, application of hyperosmotic pressure condition to the production of albuminerythropoietin (Alb-EPO) fusion protein by recombinant Chinese hamster ovary (rCHO) cells in suspension was investigated. Hyperosmotic pressure has been known to affect to the cell growth and the production of recombinant proteins. Generally, most cell lines are known to be damaged over 400 mOsm/kg. The osmotic pressure was shifted from control (320 mOsm/kg) to higher levels (380, 430, and 480 mOsm/kg) using 5 M NaCl at day 4 of cultivation. Cell growth and viability were found to be reduced with hyperosmolar condition. However, 380 mOsm/kg led to 1.2-fold increase in Alb-EPO production compared with that in control. In conclusion, hyperosmotic pressure was found to be a simple and effective parameter for increasing Alb-EPO expressed in rCHO cells.

키워드

저자

• Kyung-Ho KANG [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
• Sung-Hun CHOI [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
• Mi-Eun KONG [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
• Su-Jin KIM [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
• Joon-Serk SEO [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
• Su-Jung KIM [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
• Sun-Mi YOO [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
• Eun-Sun KO [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
• Dong-Il KIM [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576