Earticle

초록

영어

Recently, disposable bioreactors have been increasingly incorporated into preclinical, clinical, and production-scale biotechnological facilities. However, there are few studies regarding their suitability for the production of pharmaceutical proteins in transgenic plant cell cultures. Plant cell culture have been regarded as a potential alternative production system for biopharmaceuticals. In this study, two kinds of disposable bioreactors were applied to transgenic rice cell cultures for the production of recombinant human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) and they were compared with 5-L stirred tank bioreactor system. The first one is a modified wave-mixed bag with a fritted sparger, and the second is an airlift disposable bioreactor. In both systems, fed-batch culture method was used for the efficient production of hCTLA4Ig by feeding concentrated amino acids.
Dissolved oxygen level was regulated at 30% by supplementing pure oxygen with solenoid valve during the run. The results from wave-mixed and airlift disposable bioreactor showed similar maximum cell density (12.6 vs. 11.9 g DCW/L), doubling time (5.03 vs. 4.84 day) and maximum hCTLA4Ig concentration (44.5 vs. 43.7 mg/L).
However, the time needed for obtaining maximum hCTLA4Ig level was much longer in airlift disposable bioreactor (26 vs. 34 days). With higher aeration rate (0.2 vvm), 1.7-fold higher specific productivity (0.355 mg/g DCW/day) was obtained. These results show that pnumatically driven disposable bioreactor system is better for the production of recombinant proteins from plant cell cultures.

키워드

저자

• Yong Suk YANG [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
• Jun-Young KWON [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
• Su-Hwan CHEON [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
• Mi-Hee YOO [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
• Ji-Won CHOI [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
• Soo-Jin KIM [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
• Hyung-Jin NAM [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
• Dong-Il KIM [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576