Earticle

초록

영어

The mAb CO17-1A recognizes the colorectal carcinoma-associated antigen GA733-2E, which is highly expressed on the surface membrane of human colorectal carcinoma cells. We have successfully developed a plant system for production of anti-colorectal cancer monoclonal antibody, mAb CO17-1A. The C-terminal KDEL (Lys-Asp-Glu-Leu) ER retention signal might be retained the recombinant glycoprotein carrying oligomannose glycans in the ER and enhances glycoprotein accumulation. In this study, we compared the expression levels of plant-derived mAb (mAbP) CO17-1A with and without KDEL in transgenic plants. We validated the expression of mAbP CO17-1A in transgenic plants via western blot analysis, and subcellular localization was monitored under confocal microscopy. Western blot analysis showed that the expression levels of the mAbP CO17-1A with KDELwere highly expressed than in the mAbP CO17-1A without KDEL. Furthermore, in ELISA with HCL116 colorectal carcinoma cells, the expression level of the mAbP with KDEL was significantly higher than that of the mAbP without KDEL. Flow cytometry (FACS) analysis showed that the Fc domains of both purified mAbP with KDEL and mammaldan-derived mAbM had similar binding activity to the FcγRI receptor (CD64). The Fc domains of the purified mAbP without KDEL had a lower binding activity to the FcγRI receptor (CD64) than the purified mAbP with KDEL. Glycosylation analysis revealed that mAbP CO17-1A without KDEL contained plant-specific N-glycans. On the other hand, mAbP CO17-1A with KDEL contained mainly oligomannose type glycans. Taken together, these results suggest that the ER retention signal peptiede (KDEL) can affects expression and glycan structures of mAbs.

키워드

저자

• Yangkang SO [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Chonbuk, 570-749. ]
• Kyung-Jin LEE [ Korean Research Institute of Bioscience & Biotechnology, Daejeon 305-806. ]
• Da-Young PARK [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Chonbuk, 570-749. ]
• Jung-Kwan LEE [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Chonbuk, 570-749. ]
• Seung Han OH [ Department of Agricultural Biology, College of Agriculture and Life Science, Chonnam National University, Gwangju. ]
• Yeon Soo HAN [ Department of Agricultural Biology, College of Agriculture and Life Science, Chonnam National University, Gwangju. ]
• Youngwan KIM [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Chonbuk, 570-749. ]
• Kisung KO [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Chonbuk, 570-749. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576