Joon-Serk SEO, Sung-Hun CHOI, Su-Jin KIM, Mi-Eun KONG, Kyung-Ho KANG, Su-Jung KIM, Sun-Mi YOO, Dong-Il KIM
언어
영어(ENG)
URL
https://www.earticle.net/Article/A129573
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
In recombinant Chinese hamster ovary (rCHO) cell cultures, a particular attention has been paid to the design of an optimized medium in order to obtain high cell densities and enhanced productivity of recombinant proteins. For the achievement of these objectives, addition of various adding nutrient supplement was attempted and many results showed that the addition of various hydrolysates has been effective to enhance productivity. In this study, we added 3 g/L of yeast extract and phytopetone at day 3 to enhance the cell growth and production of albumin-erythropoietin(alb-EPO) supported maximum in rCHO cells. When we added the hydrolysates, it was found that yeast extract supported maximum cell density as well as alb-EPO concentration. Improvement of cell growth and alb-EPO production was 1.4-and 1.5-fold respectively. Lactate production, which is related with glucose consumption, was 2.4-fold lower than that of control culture without hydrolysates. These results suggested that the cultivation of rCHO cells with the addition of yeast extract was suitable for the improvement of cell growth and alb-EPO production.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동