Earticle

초록

영어

Cryopreservation is a practical method of preserving plant cell cultures and their genetic integrity. Transgenic rice cells producing recombinant bovine trypsin were pretreated at an high osmotic condition (0.5M sucrose) to minimize formation and aggregation of ice during freezing.
Transgenic rice cells were slowly prefreezing using Mr. Frosty freezing container in a deep freezer (-70℃) and preserved in liquid nitrogen (-196℃). For development of an optimal cryoprotectant for long-term storage, various concentrations of sucrose, glycerol and dimethyl sulfoxide (DMSO) to the precultured cells were investigated. The optimal condition of cryoprotectant was observed combination of 0.5M sucrose, 0.5M glycerol, 1M DMSO. The cell viability with this optimized condition was 62% after cell banking for 1day. The expression of recombinant trypsin in recovered callus from cryopreservation was also kept stable, and the production level was similar to that observed in noncryopreserved cultures. Acknowledgments This work was supported by a grant from the Next Generation New Technology Development program of the Ministry of Commerce, Industry and Energy, and by the National Research Foundation of Korea Grant funded by the Korean Government (2009-0070005)

키워드

저자

• Hwa-Young YU [ Dept. of Molecular Biology, Chonbuk National University, 664-14. ]
• Nan-Sun KIM [ Dept. of Molecular Biology, Chonbuk National University, 664-14. ]
• Tae-Ho KWON [ Jeonju Bio-materials Institute, Jeonbuk 561-360. ]
• Moon-Sik YANG [ Dept. of Molecular Biology, Chonbuk National University, 664-14. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576