Hyo-Boon KIM, Yun-Ji SHIN, Ju KIM, Kang-Yeol YU, Tae-Ho KWON
언어
영어(ENG)
URL
https://www.earticle.net/Article/A129566
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
Rock bream iridovirus (RBIV) have occurred frequently among cultured marine fish species in Southeast Asia (including S. Korea) and iridoviral disease has caused severe systemic disease with high mortality. The major capsid protein of RBIV is known to have a major effect on viral infection. In order to develop the edible vaccine against RBIV, MCP gene with KDEL endoplasmic reticulum retention signal was synthesized based on rice optimized codon usage (sMCP) by overlap PCR. The synthetic fragments, sMCP was inserted into a plant expression vector containing sugar starvation inducible promoter, RAmy3D, and introduced into the rice celli by bombardment-mediated transformation. The stable integration and transcriptional expression of the sMCP were confirmed by genomic DNA PCR, Northern blot analysis. Accumulation and production of major capsid protein in endoplasmic reticulum during sugar starvation in suspension-cultured cells was confirmed by Western blot analysis. These results suggest that transgenic suspension-cultured rice cells have the potential to be used as a edible vaccine against RBIV (This study was supported by Technology Development Program (108166-03-1-HD110) for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea).
키워드
Rock bream iridovirusedible vaccinesuspension-cultured rice cells
저자
Hyo-Boon KIM [ Jeonju Biomaterials Institute, Jeonju 561-360, KOREA. ]
Yun-Ji SHIN [ Jeonju Biomaterials Institute, Jeonju 561-360, KOREA. ]
Ju KIM [ Jeonju Biomaterials Institute, Jeonju 561-360, KOREA. ]
Kang-Yeol YU [ Jeonju Biomaterials Institute, Jeonju 561-360, KOREA. ]
Tae-Ho KWON [ Jeonju Biomaterials Institute, Jeonju 561-360, KOREA. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동