Earticle

초록

영어

Effects of environmental factors on cell growth and recombinant protein production have been widely investigated in Chinese hamster ovary (CHO) cell cultures. In addition, directed shifting of process parameters can be used as an optimization tool for providing higher cell and product yields. pH is a parameter known to influence cell growth, recombinant protein production, cell metabolism and protein glycosylation. In this work, we investigated the effect of pH on a recombinant CHO cell line expressing human albumin-erythropoietin (albumin- EPO), a highly glycosylated fusion protein. The culture pH was controlled at various levels (6.8, 7.0, and 7.2, respectively) by using 0.5 N NaOH.
Maximum cell density reached up to 4.2 x 106cells/mL at day 4 by maintaining pH 7.2, which was 8% higher than that of control culture. The highest productivity was obtained at pH 6.8. However, lowering the culture pH led to cell aggregation. Based on these results, pH shift two-stage culture was performed. In the first half stage of cultivation, pH was controlled at 7.2 and the pH shifted at day 5 to 6.8 and 7.0, respectively. However, as reported in the previous studies, the culture pH at 37℃ did not influence albumin-EPO production. In conclusion, the pH shifting was found to be an efficient strategy for the increase of cell growth in CHO cell cultures. Therefore, it is expected that the simultaneous use of both pH and temperature shifting can improve the performance of CHO cells regarding cell growth, recombinant protein production and glycosylation.

키워드

저자

• Sung-Hun CHOI [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
• Su-Jin KIM [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
• Mi-Eun KONG [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
• Joon-Serk SEO [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
• Kyung-Ho KANG [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
• Su-Jung KIM [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
• Sun-Mi YOO [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
• Eun-Sun KO [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
• Sang-Min LIM [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]
• Dong-Il KIM [ Dept. of Biological Engineering, Inha University, Incheon, 402-751. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576