We investigated the effects of Aurintricarboxylic Acid (ATA) on Chinese Hamaster Ovary (CHO) DG44 cell growth in serum free media with controlled calcium concentration, measuring the cell aggregation rate with the coulter counter. In previous studies, high levels of extracellular calcium ion (12.5 mg/L) showed to cause decreased cell density and high aggregation to CHO DG44 cells in serum-free media. Cell aggregation during cell culture causes hindrance of nutrient and oxygen transfer to the cell, leading to inhibition of cell growth. We observed increased cell growth and decreased cell aggregation when we added ATA in the calcium controlled medium. Exhibited effects of ATA on cell aggregation particularly appeared in high-calcium levels. In commercial serum-free media that cannot control calcium concentrations, ATA is a possible solution that may solve the cell aggregation problem in high-calcium conditions.
키워드
Aurintricarboxylic AcidATACHO cellCell aggregationSerum Free Media
저자
Se-Jung KIM [ Department of Chemical Engineering, Hanyang University, Seoul, 133-791. ]
Ui-Yeon KIM [ Department of Chemical Engineering, Hanyang University, Seoul, 133-791. ]
Hong-Woo PARK [ Department of Chemical Engineering, Hanyang University, Seoul, 133-791. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
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