Earticle

초록

영어

Porcine epidemic diarrhea virus (PEDV) is a Coronavirus and a causative agent for diarrhea in pigs, particularly in neonates. The propagation of the PEDV in cell culture was developed in 1988. Porcine epidemic diarrhea disease vaccine was produced from Vero cells by using lively attenuated virus strain[1]. The PEDV strain of PED-SM was tested in this study. The optimal MOI, infection time, and harvest time of PED-SM were 0.1, 72 hr of post-inoculation, and 24 hr of post-infection. With 0.1 MOI, PED-SM showed not only the highest virus production rate, but also highest cell death rate. Therefore, the maintenance of high cell density for long time is keenly needed for the high virus titer. A semi-perfusion culture of Vero cells was tried for the high cell density culture. The medium change with 10% FBS supplemented medium showed better cell growth than that with other concentration of FBS. However, the virus titer with medium change was lower than that with no medium change. This maybe caused by the depletion of nutrients in virus production medium due to the high cell density in semiperfusion culture. Accordingly, additional nutrients supplement maybe needed in the virus production step as well as in the cell culture step.

키워드

저자

• Chang-Jin LEE [ Dept. of Medical Biotechnology, Kangwon National University, Chuncheon, 200-701. ]
• Da-Hui HA [ Dept. of Medical Biotechnology, Kangwon National University, Chuncheon, 200-701. ]
• Yeon-Ho JEONG [ Dept. of Medical Biotechnology, Kangwon National University, Chuncheon, 200-701. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576