Eun-sun KO, Sung-hun CHOI, Mi-eun KONG, Su-jung KIM, Sun-mi YOO, Joon-serk SEO, Kyung-ho KANG, Sang-min LIM, Dong-il KIM, Chang-woo LEE
언어
영어(ENG)
URL
https://www.earticle.net/Article/A129547
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
Type 1 diabetes is caused by the complete loss of pancreatic beta cells due to the body’s own immune system. Transplantation of beta cells is considered to be the best treatment for type 1 diabetes, however it is extremely limited by the shortage of human organ donors. Due to the shortage of donor pancreata, alternative sources of islets have been studied through the generation of the beta cells from stem cells and the expansion of the beta cells with various growth factors. However, differentiation of stem cells and expansion of insulin-producing cells (IPCs) have been limited. In this study, we used periosterium-derived progenitor cells (PDPCs) known as a kind of mesenchymal stem cells (MSCs) for the differentiation into IPCs. Even though IPCs can be obtained from PDPCs, maintenance of stability and expansion of IPCs was not easy. Therefore, effects of antioxidants on the stability and expansion of IPCs were investigated in this study. Enzyme-linked immunosorbent assay (ELISA) and MTT assay were used for the measurement of stability. In addition, real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect expression levels of insulin.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동