Young Woo JANG, Bo Ram JI, Hyun Jung KIM, Chan Wha KIM
언어
영어(ENG)
URL
https://www.earticle.net/Article/A129543
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
Mesenchymal stem cells (MSCs) have a great potential in cell-based therapies because of their multipotency and simple methods for in vitro manipulation. However, during in vitro manipulation, MSCs have aging and loss their multipotency and proliferation capability. Previous studies shown that calorie restriction (CR) induces proliferation of MSCs and diminishes apoptosis. Therefore, we observed the effect of low glucose in MSC in vitro. Proliferation level in the low glucose (LG, 1.375 mM) condition was compared with that in the normal glucose (NG, 5.5 mM) condition. We performed comparative studies of population doubling, β-galactosidase activity, ROS generation and differentiation capacity (osteocyte, adipocyte) in NG and LG conditions. We compared protein expressions between NG and LG conditions, several proteins were up-regulated or down-regulated in glucose restriction condition, LG condition. These results will be expected to provide a powerful tool for long-term and large scale culture of stem cells.
키워드
MSCcalorie restrictioncell culture
저자
Young Woo JANG [ School of Life Sciences and Biotechnology, Korea University. ]
Bo Ram JI [ School of Life Sciences and Biotechnology, Korea University. ]
Hyun Jung KIM [ School of Life Sciences and Biotechnology, Korea University. ]
Chan Wha KIM [ School of Life Sciences and Biotechnology, Korea University. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동