Mi-Eun KONG, Sung-Hun CHOI, Su-Jin KIM, Su-Jung KIM, Sun-Mi YOO, Joon-Serk SEO, Kyung-Ho KANG, Eun-Sun KO, Dong-Il KIM
언어
영어(ENG)
URL
https://www.earticle.net/Article/A129540
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
PCT15 Tissue engineering and regenerative medicine are new research areas investigating how to repair and regenerate impaired organs and tissues using the natural signaling pathway and components of the organism. In the case of joint cartilage, there are no blood vessels and the damaged tissues are not usually regenerated by themselves. For the treatment of damaged cartilage or initial symptom of osteoarthritis, transplant surgery should be carried out with scaffold beads to support the cells transplanted. In this study, the possibility of a manufactured HA-atelo scaffold bead as a support material for cartilage cell therapy was investigated. We compared the manufactured HA-atelo scaffold beads with two other types of commercial scaffold beads such as Cellgen and Cytodex3. The HA-atelo scaffold beads were manufactured through a five-step process including HA dilution, collagen mixture, bead formation, washing, and bead collection. Isolated chondrocytes are cultured and compared in three types of scaffold beads. Cell proliferation was determined by using MTT assay and secreted glycosaminoglycan (GAG) was analyzed quantitatively by using Blyscan GAG assay kit. In conclusion, the manufactured HA-atelo scaffold beads provided the best results for the cultivation of chondrocytes and they can be used in cartilage tissue engineering effectively.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동