Mesenchymal stem cells (MSCs) derived from human umbilical cord blood (hUCB) represent encouraging candidates for the development of future cellular therapy strategies. MSCs have been found to have pluripotent which is able to differentiate into various tissues. One of the primary limitations in our study of the biology of human MSCs is the absence of prospective markers required for the monitoring of lineage-specific differentiation. hUCB-derived MSCs have been found to have significantly greater osteogenic potential. In this study, we focused on protein expression changes during osteogenic differentiation of hUCB-MSCs (n=3). And we analyzed the protein expression inherent to osteogenic differentiation by proteomic research including two-dimensional gel electrophoresis, ESI-Q-TOF, and Western blotting. Eleven differentially expressed proteins including β-actin, pyridoxal phosphatase, PGAM1, VBP1, Hsp27 and HSPC231 were confirmed between the two groups (before and after differentiation). These might also be proved as useful cytosolic biomarkers for osteogenesis, and might be employed in quality control of osteoblasts in cell-therapy applications.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
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