Sun-Mi YOO, Sung-Hun CHOI, Mi-Eun KONG, Su-Jin KIM, Su-Jung KIM, Joon-Serk SEO, Kyung-Ho KANG, Eun-Sun KO, Sang-Min LIM, Dong-Il KIM, Chang-Woo LEE
언어
영어(ENG)
URL
https://www.earticle.net/Article/A129479
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
The transplantation of pancreatic islet cells is a typical method for the treatment of type I diabetes. The treatment is limited by the shortage of islet cells, which can produce insulin in vivo. Various kinds of stem cells can be used for the preparation of insulin-producing cells (IPCs) such as adipocyte-derived mesenchymal stem cells (MSCs), bone marrow-derived MSCs and embryonic stem cells. In order to obtain IPCs from MSCs effectively, differentiation conditions should be optimized properly. In this study, periosteum-derived progenitor cells (PDPCs) from independent donors were used for the preparation of IPCs. PDPCs have been known to be another kind of MSCs and are expected to be used as a cell source to make IPCs. Homologous PDPCs was obtained by sorting from each donor’s periosteum-derived cells (PDCs) of cambium layer by fluorescent activated cell sorter (FACS) using surface markers of MSCs such as CD9, CD90, and CD166. For the differentiation into IPCs, PDPCs were cultured for 2 weeks in serum-free DMEM/F12 with several supplements containing activin A, exendin 4, nicotinamide, hepatocyte growth factor. It was confirmed that IPCs could be obtained efficiently by the differentiation of PDPCs using several analytical methods including PCR, real-time PCR, immunofluorescence, and ELISA.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동