Water hyacinth has many advantages to product bioethanol, because it grows fast and is not edible [1]. Alkaline/oxidative pretreatment A/O pretreatment) using NaOH/H2O2 solution is known as most effective pretreatment method of water hyacinth [2]. In this study, we optimized A/O pretreatment conditions (solution concentration, reaction temperature) and analyzed component of each pretreatment sample for investigating effect of A/O pretreatment in many condition. In order to optimize the hydrolysis condition, Celluclast and Viscozyme 1.5L were applied at various enzyme concentrations and enzyme ratios. Ethanol production was performed using SHF, SSF and batch fermentation mode by Saccharomyces cerevisiae KCTC 7928. As a result, the most effective A/O pretreatment condition is NaOH concentration 7% (w/v) at 100℃ and H2O2 concentration 2%. Amount of glucose concentration is 18.29 (g/l). The yield of ethanol production was 0.33 (g/g- pretreatment). The concentration of ethanol was analyzed by HPLC with RI detector (flow rate 0.6ml/min, oven temperature 40℃).
키워드
water hyacinthbioethanolpretreatment
저자
Deuk Joo AHN [ Dept. of Biological Engineering, Inha University, Inchoen, 402-751, Korea. ]
Hyun Shik YUN [ Dept. of Biological Engineering, Inha University, Inchoen, 402-751, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동