Earticle

초록

영어

In a previous study, we co-cultured the novel strain Bacillus licheniformis SGP1 with Clostridium tyrobutyricum ATCC 25755 for butyric acid production using sucrose as a carbon source in RCM medium. In this co-culture, B. licheniformis produces levansucrase enzyme in the medium which degrades sucrose into monosaccharides allowing C. tyrobutyricum to utilize them along with the lactic acid produced by B. licheniformis for butyric acid production. In this study, we wanted to determine the effect of different factors on the growth of this novel Bacillus strain and its levansucrase production and activity. Optimum pH was around 7.0. Optimum temperature for growth is 37⁰C. Enzyme production is decreased dramatically as the temperature rises from 30⁰C to 43⁰C and the reverse happens for enzyme activity. This enzyme has a molecular weight of 55 KDa. In fed-batch fermentation at pH 5.50 and temperature of 37⁰C, the co-culture could give a final butyrate concentration of 36 g/L with productivity equals 0.24 g/L/hr, while at 40⁰C, we could get 28.6 g/L and 0.34 g/L/hr respectively. Real time PCR experiment was done finally to determine the growth pattern of the two strains in the co-culture.

키워드

저자

• Mohammed DWIDAR [ Clean Energy Center, Korea Institute of Science and Technology (KIST). Department of Green Chemistry and Environmental Biotechnology, University of Science and Technology (UST). ]
• Se-il KIM [ Clean Energy Center, Korea Institute of Science and Technology (KIST). ]
• Byoung Seung JEON [ Clean Energy Center, Korea Institute of Science and Technology (KIST). ]
• Youngsoon UM [ Clean Energy Center, Korea Institute of Science and Technology (KIST). ]
• Byoung-In SANG [ Clean Energy Center, Korea Institute of Science and Technology (KIST). Department of Green Chemistry and Environmental Biotechnology, University of Science and Technology (UST). ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576