Jung Kon KIM, Bon-Cheol KOO, Young-Lok CHA, Gi Hong AN, Seung-Hyun AHN, Surn-Teh BARK, Youn-Ho MOON, Young-Hwan CHOI, Sae-Jung SUH
언어
영어(ENG)
URL
https://www.earticle.net/Article/A129440
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원문정보
초록
영어
From 21 strains, which is isolated from various environmental samples, D1 and E2 microbial strains were characterized and identified as Bacillus subtilis by analysis of 16S rDNA sequence and biochemical studies, and named as B. subtilis D1 and E2, which have high cellulase activities. The cellulase gene was cloned from each B. subtilis D1 and E2 genomic DNA by polymerase chain reaction (PCR). The amplified PCR products were ligated with the T&A cloning vector system and the constructed plasmids were transformed into E. coli DH5α. The sequence analysis of the insert DNAs revealed the identification of a 1,499-bp region containing cellulase open reading frame. According to cellulase gene sequence analysis, B. subtilis D1 had gene sequence similarity of 98% with B. subtilis strain AH18 cellulase gene (EF070194.1) and B. subtilis I15-endo-1,4-beta-glucanase (FJ464332.1). In case of B. subtilis E2, it had gene sequence similarity of 99% with B. subtilis strain AH18 cellulase gene (EF070194.1) and B. subtilis CMCase gene (D01057.1).
키워드
CellulaseBioenergyBacillus subtilis
저자
Jung Kon KIM [ Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, Muan, Jeonnam, 534-833. ]
Bon-Cheol KOO [ Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, Muan, Jeonnam, 534-833. ]
Young-Lok CHA [ Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, Muan, Jeonnam, 534-833. ]
Gi Hong AN [ Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, Muan, Jeonnam, 534-833. ]
Seung-Hyun AHN [ Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, Muan, Jeonnam, 534-833. ]
Surn-Teh BARK [ Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, Muan, Jeonnam, 534-833. ]
Youn-Ho MOON [ Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, Muan, Jeonnam, 534-833. ]
Young-Hwan CHOI [ Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, Muan, Jeonnam, 534-833. ]
Sae-Jung SUH [ Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, Muan, Jeonnam, 534-833. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동