Earticle

초록

영어

The medium composition for butanol productivity by Clostridium pasteurianum DSM525 was optimized using response surface methodology (RSM). A fractional factorial design (FFD) for eight variables (phosphate buffer, MnSO4·H2O, MgSO4·7H2O, FeSO4·7H2O, (NH4)2SO4, yeast extract, cystein and MES) was applied to identify which variables affected butanol production. Among them, FeSO4·7H2O, (NH4)2SO4 and yeast extract were identified to be the most significant factors. Box-Behnken Model (BBM) was applied to estimate their values for the optimal medium compositions for butanol productivity. The composition of optimal medium for butanol productivity by C. pasteurianum DSM525 was determined to be 1.5 g/L of K2HPO4, 1.5 g/L of KH2PO4, 0.02 g/L of MnSO4·H2O, 0.25 g/L of MgSO4·7H2O, 0.06 g/L of FeSO4·7H2O, 7.4 g/L of (NH4)2SO4 and 5.0 g/L of yeast extract. The predicted butanol productivity by RSM (0.98 gL-1h-1) was verified by experiment using the optimal medium (1.01 gL-1h-1).
Considering that the butanol productivity with the modified P2 (MP2) medium, a general butanol fermentation medium, was 0.71 gL-1h-1, butanol productivity was enhanced by 40% with the optimized medium composition resulted from RSM analysis.

키워드

저자

• Youngsoon UM [ Center for Environment Technology Research, Korea Institute of Science and Technology, Seoul, Korea. ]
• Chuloo MOON [ Center for Environment Technology Research, Korea Institute of Science and Technology, Seoul, Korea. ]
• Kyung Duk KIM [ Center for Environment Technology Research, Korea Institute of Science and Technology, Seoul, Korea. ]
• Byoung-In SANG [ Center for Environment Technology Research, Korea Institute of Science and Technology, Seoul, Korea. ]
• Seung Wook KIM [ Department of Chemical and biological, Korea University, Seoul, Korea. ]

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576