Several agar-degrading bacteria were isolated from gut of spiny turban shells. These bacteria were examined by Lugol’s iodine and identified as extracellular agarase-producing bacteria. Among them, one species made clear depressions around colonies. Agar-degrading ability of this bacterium was examined by measuring viscosity-change of the inoculated medium. The viscosity of the inoculated medium was decreased by more than 60% after 20 hour-cultivation while pure medium did not show any decline of viscosity. The sequence of 16s rRNA of the bacterium was analyzed and a comparative analysis of the 16s rRNA sequence was performed by BLASTN with Genebank data base. The result indicated that it might be one species of the genus, Pseudoalteromonas. In addition, a homology-comparison-study of relative strains was performed, and as a result, the bacterium was identified as one of Pseudoalteromonas nigrifaciens strains. Experiments, such as assay of enzyme activity, enzyme identification are being performed for further study.
키워드
IsolationAgarDegrading
저자
Young Hoon OH [ Dept. of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742. ]
Changkyou JUNG [ Dept. of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742. ]
Jinwon LEE [ Dept. of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
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