Earticle

초록

영어

Agar is extracted from red seaweeds and it is an important gelifying agent for biochemical use especially in the food industry. The enzymatic breakdown of agar can be performed by two types of agarases. Neoagaro-oligosaccharides produced by β-agarase inhibit the growth of bacteria, slow down the rate of degradation of starch, and are used as low-calorie additives to improve food quality. Therefore the development of the agarolytic complex capable of more effective agar hydrolysis ability is required to bring about a dramatic reduction of production costs. In this study, we expressed recombinant scaffolding protein mini-CbpA from Clostridium cellulovorans. To assemble the agarolytic complex via cohesin-dockerin interaction, we constructed a chimeric agarase cAgaB from Zobellia galactonovorans containing the catalytic domain of AgaB fused with a tandem-aligned dockerin domain of C. cellulovorans EngB. The resulting hydrolysis complex could degrade agar efficiently with synergic effect of agar-degrading enzyme activities. This is the first report on the formation of agarolytic complex using the cohesin-dockerin interaction system. The enhancement of agar-degrading complex activities will lead to the commercial production of useful products from agar biomass at low costs.

키워드

저자

• Sung Ok HAN [ School of Life Sciences and Biotechnology, Korea University. ]
• Jeong Eun HYEON [ School of Life Sciences and Biotechnology, Korea University. ]
• Sun Young WHANG [ School of Life Sciences and Biotechnology, Korea University. ]
• Woo Jung JEON [ School of Life Sciences and Biotechnology, Korea University. ]

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간행물

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• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576