Earticle

초록

영어

Mussel adhesive proteins (MAPs) have been regarded as outstanding marine-derived biomaterials due to their biocompatibility, biodegradability, and adhesion ability to various surfaces. In order for MAP to crosslink and adsorb quickly in the tough sea environment, tyrosine residues of MAP are modified into 3,4-dihydroxyphenyl-L-alanines (L-DOPAs) by natural mechanism. In our previous research, fp-151, a recombinant MAP, was successfully over-expressed as a form of inclusion body in Escherichia coli. However, in vitro tyrosine modification was demanded after production for higher adhesion characteristic of fp-151.
Instead of performing this additional treatment, in the present work, E. coli co-expression system of tyrosinase and fp-151 was constructed to modify tyrosine residues in vivo. In this method, fp-151 was over-expressed and mainly obtained not from inclusion body, but from soluble fraction. Furthermore, MALDI-MS/MS analysis of solubleexpressed fp-151 implies some of tyrosine residues were definitely modified. This study suggested one possible strategy for in vivo incorporation of modified tyrosine residues in MAPs. The approach is applicable for economic and efficient modification of tyrosine residues.
Yoon Jeong YANG, Dooyup JUNG, Yoo Seong CHOI and Hyung Joon CHA*
Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, 790-784, Korea.
PMB03 Comparison of Lipid Contents of Chlorella sp. MB01 and Chlorella sp. MB02 Cultivated in Various Media

키워드

저자

• Yoon Jeong YANG [ Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, 790-784, Korea. ]
• Dooyup JUNG [ Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, 790-784, Korea. ]
• Yoo Seong CHOI [ Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, 790-784, Korea. ]
• Hyung Joon CHA [ Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, 790-784, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576