Currently, there is increasing interests about health drinks such as fruit juices and plant extracts prepared with heat under pressure. However, fruit juices and plant extracts contain high quantity of sucrose. After drinking, the sucrose remains in mouth may cause tooth decay and obesity by high-calories. To make low-calorie and sweet juices and extracts,, we developed method to make new functional fruit juices and plant extracts having sucrose concentration and high content of oligosaccharides by using dextransucrase prepared from Leuconostoc mesenteroides. In case of orange juice, 8.9 U/ 100 ml of dextransucrase was added and reacted at 16°C for 20 hr. The sucrose content was reduced from 39.6% to 6.02% and oligosaccharide content increased up to 41.6% in juice. In case of arrowroot extract, 16.8 U/ 100 ml of dextransucrase was added and incubated at 16°C for 4 hr. The sucrose content was changed from 56.63% to 5.77% and oligosaccharide content became 65.87% in extract. The enzyme reacted orange juice and arrowroot extract showed 91.15% and 89.60% inhibition effect compared to control (the original orange juice and arrowroot extract), respectively, for the insoluble glucan formation by mutansucrase produced from cariogenic Streptococcus mutans. From this result, the newly synthesized functional fruit juices and plant extracts containing low sucrose concentration and high content of oligosaccharides may contribute in positive for dental health. Studies about the prebiotic effects of those new fruit juices and plant extracts are in progress and will be discussed during symposium.
키워드
OligosaccharidesDextransucrasefruit juice and plant extracts
저자
Hye-Jin WOO [ School of Biological Sciences and Technology and The Research Institute for Catalysis, Chonnam National University, Gwang-Ju, 500-757, South Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동