Earticle

초록

영어

Hydroquinone galactoside (HQ-Gal) as a potential skin whitening agent was synthesized by the reaction of lactase (β-galactosidase) from Kluyveromyces lactis, Aspergillus oryzae, Bacillus circulans and Thermus sp. with lactose as a donor and HQ as an acceptor. Among these lactases, the acceptor reaction involving HQ and lactose with K. lactis lactase showed a higher conversion ratio to HQ-Gal (60.27%). HQ-Gal was purified using butanol partitioning and silica-gel column chromatography. The structure of the purified HQ-Gal was determined by nuclear magnetic resonance and the ionic product was observed at m/z 295 (C12H16O7 Na)+ using matrix assisted laser desorption ionization time-of-flight mass spectrometry.
HQ-Gal was identified as 4-hydroxyphenyl-β-D-galactopyranoside. The optimum conditions for HQ-Gal synthesis by K. lactis determined using response surface methodology were 50 mM HQ, 60 mM lactose and 20 U mL-1 lactase. These conditions produced a yield of 2.01 g L-1 HQ-Gal. The half maximal inhibitory concentration (IC50) of diphenylpicryl-hydrazyl scavenging activity was 3.31 mM indicating a similar anti-oxidant activity compared to β-arbutin (IC50 = 3.95 mM). The Ki value of HQ-Gal (0.75 mM) against tyrosinase was smaller than that of β-arbutin (Ki = 1.97 mM), indicating its superiority as an inhibitor. HQ-Gal inhibited (23%) melanin synthesis without being significantly toxic to the cells, while β-arbutin exhibited only 8% reduction of melanin synthesis in B16 melanoma cells compared with the control.
These results indicate that HQ-Gal may be a suitable functional component in the cosmetics industry.

키워드

저자

• Sun Hwa JUNG [ Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials Chonnam National University, Gwangju 500-757. ]
• Go Eun KIM [ Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials Chonnam National University, Gwangju 500-757. ]
• Jin Ha LEE [ Dental Science Research Institute Chonnam National University, Gwangju 500-757. ]
• Eun Seong SEO [ School of Biological Sciences and Technology and Research Institute for Catalysis, Chonnam National University, Gwangju 500-757. ]
• Sheng De JIN [ School of Biological Sciences and Technology and Research Institute for Catalysis, Chonnam National University, Gwangju 500-757. ]
• Ghahyun J KIM [ Department of Biology, University of California San Diego, La Jolla 92093, USA. ]
• Jaeho CHA [ Department of Microbiology, Pusan National University, Busan 609-735, ]
• Eui Joong KIM [ GenoFocus Inc., Daejeon 305-811. ]
• Ki-Deok PARK [ Korea Basic Science Institute, Gwangju 500-757, Republic of Korea. ]
• Doman KIM [ Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, School of Biological Sciences and Technology and Research Institute for Catalysis, Chonnam National University, Gwangju 500-757. ]

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발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576