Myoung Eun JUNG, Jinhyeok LEE, Haejin KIM, Jung Im LEE, Youngwan SEO
언어
영어(ENG)
URL
https://www.earticle.net/Article/A129399
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
As a part of search for antioxidants from marine organisms, antioxidant activities of two seagrasses Zostera japonica and Z. asiatica were investigated. Each of them was extracted two times with acetone-CH2Cl2(1:1) and MeOH in turn. Crude extracts were combined for each sample. Each of the combined crude extracts was partitioned between CH2Cl2 and water. The organic layer was further partitioned between 85% aq MeOH and n-hexane, and the aqueous layer was fractionated with n-BuOH and H2O, successively. The protective effect of each fraction from Z. japonica and Z. asiatica on H2O2-induced oxidative stress was examined in Raw 264.7 cells. The levels of intracellular ROS and GSH were measured using 2',7'-dichlorofluorescin diacetate and monobromobimane as fluorescence probe, respectively. All solvent fractions including their crude extracts not only significantly decreased levels of intracellular ROS in a dose-dependent manner but also increased intracellular GSH level. Moreover, they exhibited a significant protective effect on membrane lipid peroxidation in TBARS assay system. These results suggest that potential natural antioxidants related to oxidative stress could be isolated from Z. japonica and Z. asiatica
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동