Optimal conditions for functional expression of a single-chain antibody (scFv) against B-type natriuretic peptide in methylotrophic yeast Pichia pastoris.
The yeast pichia pastoris has been well known as a highly successful system for the production of various recombinant heterologous proteins. To improve scFv production in pichia pastoris, parameters such as cultivation temperature, initial pH of medium and cell density were examined. Investigation of culture conditions revealed that the maximal cell growth and functional scFv expression were achieved at pH 6.0-7.0 and at 20 ℃ which is relatively low temperature. The N-terminal sequence of expressed anti-BNP scFv was analyzed and two Ste13 sites were not cut out at all unlike our expectation. However the function or activity of anti-BNP scFv was not affected significantly. The optimized culture conditions determined through this study will be used from the efficient production of anti-BNP scFv.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동