Byoung Hoon YOON, Woo Young JEON, Woo Yong SHIM, Jung Hoe KIM
언어
영어(ENG)
URL
https://www.earticle.net/Article/A129375
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원문정보
초록
영어
Many yeasts and fungi can assimilate L-arabinose via a complex pathway consisting of two reduction, two oxidation and phosphorylation. Unspecific aldose reductase catalyzes the reduction of L-arabinose to L-arabitol, and L-arabitol dehydrogenase (LAD) oxidizes L-arabitol to L-xylulose. This is subsequently reduced to xylitol by L-xylulose reductase (LXR). After that, xylitol dehydrogenase (XDH) catalyzes the oxidation of xylitol to D-xylulose. D-xylulose is converted to D-xylulose 5-phosphate by xylulose kinase (XK) and then enters the pentose phosphate pathway (PPP). However, Candida tropicalis ATCC 20336 cannot grow on media containing L-arabinose as the sole carbon, which indicates that there is not L-arabinose metabolic pathway in this strain. For consumption of L-arabinose via PPP, bacterial L-arabinose metabolic pathway was introduced to C. tropicalis. Bacillus licheniformis L-arabinose isomerase (araA, AI), E. coli L-ribulokinase (araB, RK) and L-ribulose-5-phosphate 4-epimerase (araD, RPE) were selected and backtranslated using the preferred codons in C. tropicalis. Codon-optimized genes were synthesized and expressed in C. tropicalis. The resulting recombinant yeast expressed araBAD successfully and survived utilizing L-arabinose as a sole carbon source.
키워드
Candida tropicalisArabinosearaBAD
저자
Byoung Hoon YOON [ Dept. of Biological Sciences, KAIST, Daejeon, Korea. ]
Woo Young JEON [ Dept. of Biological Sciences, KAIST, Daejeon, Korea. ]
Woo Yong SHIM [ Dept. of Biological Sciences, KAIST, Daejeon, Korea. ]
Jung Hoe KIM [ Dept. of Biological Sciences, KAIST, Daejeon, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동