Earticle

초록

영어

Hydrogen production in biological way can be one of solutions for energy crisis. Previously, we successfully demonstrate to produce biohydrogen by expressing [NiFe]-hydrogenase in recombinant E. coli.
Although [NiFe]-hydrogenase showed relatively high oxygen-tolerance compare to other hydrogenases, still biohydrogen production efficiency was low. In this work, we introduce proteorhodopsin, light acceptor for prokaryote working as proton pump, into [NiFe]-hydrogenase expressing recombinant E. coli for using light energy to produce biohydrogen.
Proteorhodopsin need a chemical named retinal for working as proton pump by using light energy but E. coli does not have a pathway to synthesize it. In order to give E. coli a ability to synthesize retinal, we express five foreign genes expressing enzymes for retinal synthesize pathway. By introducing light accepting system into E. coli expressing [NiFe]-hydrogenase, we could get enhanced biohydrogen productivity.

키워드

저자

• Younghwa JO [ Department of Chemical Engineering, Pohang University of Science and Technology, Pohang. ]
• Jaoon Y.H. KIM [ Department of Chemical Engineering, Pohang University of Science and Technology, Pohang. ]
• Hyung Joon CHA [ Department of Chemical Engineering, Pohang University of Science and Technology, Pohang. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576