Earticle

초록

영어

The objective of this research is to enhance the hydrogen productivity in fermentative hydrogen producing bacterium; E. asburiae SNU-1 by genetic manipulation. In certain formate concentration and pH, formate hydrogen lyase (FHL) decomposes formate to hydrogen and CO2. This FHL is controlled by fhlA transcription activator and hycA repressor.
Consequently, to increase the FHL, the aim is the overexpression of fhlA gene and deletion of hycA gene. From results of SDS-PAGE and Western-blot, we confirmed the fhlA overexpression in E. asburiae SNU-1. hycA gene was disrupted by Red/ET recombination and it was confirmed by antibiotics resistance tests and colony PCR where several primer combinations were used. We tested the hydrogen productivity in PYG growth medium and in formate production medium using ΔhycA and fhlA overexpressed E. asburiae SNU-1, and found that fhlA overexpression has specific hydrogen production enhancing effect by 36.89% and 56.28%, respectively. But unfortunately ΔhycA shows reduction in hydrogen productivity. These results show that the overexpression of fhlA has hydrogen production enhancing effect and hence anticipate its use in hydrogen production systems.

키워드

저자

• Juneok LEE [ School of Chemical and Biological Engineering. ]
• Jong-Hwan SHIN [ Interdisciplinary Program, Bioengineering Major, Graduate School. ]
• Jung Hyuk KANG [ Interdisciplinary Program, Bioengineering Major, Graduate School. ]
• Hyung Lae LEE [ Interdisciplinary Program, Bioengineering Major, Graduate School. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576