Earticle

초록

영어

LK8 protein is the 38th kringle fragment of human apoliprotein consisting of 86 amino acids with strong anti-angiogenesis activity.
Invertase is a glycoprotein that catalyzes the hydrolysis of sucrose into fructose and glucose. With these two model proteins, we investigated the effect of the various folding or secretory pathway associated proteins in Saccharomyces cerevisiae. First, to establish the stable and efficient expression system, LK8 and invertase expression cassettes containing the galactose-induced promoter and mating factor-α signal sequence were constructed and introduced into S. cerevisiae 2805 by δ-integration. Second, to facilitate protein maturation and exit from ER, several folding accessories, Pdi1P, Sec23p and Trx2p were coexpressed with LK8 or invertase. Pdi1p, Sec23p and Trx2p increased the secretion of LK8 from 18.1 mg/L to 23.7, 20.7 and 19.3 mg/L, respectively. In Pdi1p, Sec23p and Trx2p coexpressed strains, the invertase activity in media was 70.2, 65.7 and 47.1 U/mL, respectively, whereas the control strain showed 47.5 U/mL of activity. Sec23p was effective in both cases as well as Pdi1p, a well-known protein folding facilitator.

키워드

저자

• Hyung-Rho LEE [ Department of Agricultural Biotechnology, Seoul National University, Seoul, Korea. ]
• Yu-Mi KIM [ Department of Agricultural Biotechnology, Seoul National University, Seoul, Korea. ]
• Yi-Hyun BAE [ Department of Agricultural Biotechnology, Seoul National University, Seoul, Korea. ]
• Hyung-Kweon LIM [ Mogam Biotechnology Research Institute, Yongin, Kyunggi, Korea. ]
• Yong-Cheol PARK [ Department of Advanced Fermentation Fusion Science and Technology, Kookmin University, Seoul, Korea. ]
• Jin-Ho SEO [ Department of Agricultural Biotechnology, Seoul National University, Seoul, Korea. ]

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발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576