So Yeong BAHN, Yoo Seong CHOI, Bong-Hyuk CHOI, Bum Jin KIM, Hyung Joon CHA
언어
영어(ENG)
URL
https://www.earticle.net/Article/A129355
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원문정보
초록
영어
Gene therapy is considered as a potential remedy for treating diseases. Therefore, safe and efficient gene delivery materials have been investigated. Gene delivery vectors are categorized into two groups: viral and non-viral. Though viral vectors are most effective, safety concerns are limitations on clinical cure. However, non-viral vectors have improved safety and manufacturing capacity. Here, we report the recombinant mussel adhesive protein (MAP) as a synthetic DNA delivery vehicle. Recombinant MAPs are biocompatible, biodegradable proteins for underwater adhesion. Because of the plenty of basic amino acid composition, they were considered as a possible carrier of DNA. After DNA binding assay, mouse NIH3T3 cells were transfected with pEGFP by MAP. The transfection efficiency was compared with LipofectamineTM 2000, the widespread transfection agent. Optimized transfection condition will achieve better capability in transferring DNA into model cell line.
So Yeong BAHN [ School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, 790-784. ]
Yoo Seong CHOI [ Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, 790-784. ]
Bong-Hyuk CHOI [ Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, 790-784. ]
Bum Jin KIM [ School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, 790-784. ]
Hyung Joon CHA [ School of Interdisciplinary Bioscience and Bioengineering and Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, 790-784. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동