Earticle

초록

영어

Succinic acid has been of interest with its various uses as a precursor for industrially useful chemicals, pharmaceuticals, and biopolymers. Native succinic acid producers including Mannheimia succiniciproducens were found to produce phosphoenolpyruvate (PEP) carboxykinase as a main catalyst for the carboxylation of PEP yielding oxaloacetate, a key intermediate for succinic acid1. Hence, we employed the PEP carboxykinase from M. succiniciproducens to a metabolically engineered E. coli strain which generated succinic acid with a high yield by increasing PEP pool under the anaerobic condition. In addition to the introduction of the enzyme, we also constructed the genetic environment to amplify the effect of PEP carboxykinase. Consequently, we observed the advantages of using PEP carboxykinase originated from M. succiniciproducens in a non-natural succinic acid producer and developed enhanced succinic acid producing E. coli strain in the aspect of both productivity and yield while reducing byproducts. [This work was supported by the Genome-Based Integrated Bioprocess Development Project of the Ministry of Education, Science and Technology (MEST) through the National Research Foundation of Korea (#20100002111). Further supports by WCU (World Class University) program by MEST (R32-2008-000-10142-0) are appreciated.]

키워드

저자

• Kyung Yun LEE [ Dept. of Chemical & Biomolecular Engineering, KAIST, Daejeon 305-701, Republic of Korea. ]
• Eun Jung CHOI [ Dept. of Chemical & Biomolecular Engineering, KAIST, Daejeon 305-701, Republic of Korea. ]
• Jeong Wook LEE [ Dept. of Chemical & Biomolecular Engineering, KAIST, Daejeon 305-701, Republic of Korea. ]
• Yu-Sin JANG [ Dept. of Chemical & Biomolecular Engineering, KAIST, Daejeon 305-701, Republic of Korea. ]
• Sang Yup LEE [ Dept. of Chemical & Biomolecular Engineering, KAIST, Daejeon 305-701, Republic of Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576