Earticle

초록

영어

It has been demonstrated that the approach of enzyme coatings on various nanomaterials is successful in stabilizing the enzyme activity in an unprecedented way [1,2,3]. For example, the trypsin coatings on electrospun polystyrene-poly(styrene-co-maleic anhydride)(PS-PSMA) nanofibers showed no decrease of enzyme activity under recycled uses and rigorous shaking for one year [3]. In the present work, trypsin was covalently attached or coated onto PS-PSMA nanofibers with varied concentrations of maleic anhydride group, which enables an easy conjugation of enzymes onto nanofibers. The concentration of maleic anhydride group correlated well with the enzyme loading and activity of covalently-attached trypsin. Trypsin-coated nanofibers, prepared via covalent attachment and follow-up enzyme crosslinking, resulted in highly stable nanobiocatalytic systems, irrespective of added PSMA amounts. The variation of PSMA amounts enables the facile control of various properties of trypsin-coated nanofibers, such as enzyme loading, activity, structure, and mass transfer limitation. The property control of highly stable enzyme coatings would provide a powerful tool for more successful applications of enzyme coatings in various fields.

키워드

저자

• Hyo Jin AN [ Dept. of Chemical and Biological Engineering, Korea University, Seoul, 136-701. ]
• Sang-Mok LEE [ Center for the Advanced Bioseparation Technology, Inha University, Incheon, 402-751. ]
• Seung-Hyun JUN [ Dept. of Chemical and Biological Engineering, Korea University, Seoul, 136-701. ]
• Yoon-Mo KOO [ Center for the Advanced Bioseparation Technology, Inha University, Incheon, 402-751. ]
• Jungbae KIM [ Dept. of Chemical and Biological Engineering, Korea University, Seoul, 136-701. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576