Cadaverine can be used a monomer for a biobased polyamide with dicarboxylate such as adipic acid. Latest research environment has driven the sustainability as a major goal of chemical production in next generation. In such a viewpoint, production of polymers using cadaverine, which can be produced from L-lysine, is highly preferred. In this work, we researched the screening of highly active lysine decarboxylase. L-lysine was used as limiting C-source and N-source in minimal media culture. As a result, we have screened Achromobacter sp. which is from soil source and shows high activity in the a-decarboxylation reaction of L-lysine. In whole cell reaction, A. sp. showed 4.5x10-3μmol/min×mg(dry cell weight) while Escherichia coli K-12 showed 2.6x10-3 μmol/min×mg(dcw). Optimum pH and temperature of lysine decarboxylase reaction using A. sp was pH 5.8 and 30℃, respectively.
키워드
lysine decarboxylasecadaverineachromobacter sp.
저자
Ahram CHAE [ Interdisciplinary Program of Bioengineering, Seoul National University, Seoul, 152-742. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
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