Earticle

초록

영어

Two enzyme mixtures of α-l-arabinofuranosidase and endo-1,5-α-l-arabinanase from C. saccharolyticus produced l-arabinose from debranched arabinan or sugar beet arabinan. The optimum ratio for debranched arabinan was α-l-arabinofuranosidase(42 U ml–1) and endo-1,5-α-l-arabinanase (14 U ml–1) of 3:1 at pH 6.5 and 75 ºC for 1 h.
Under the optimum ratio, 16 g l–1 l-arabinose was obtained from 20 g l–1 debranched arabinan after 1h, with a conversion yield of 80% and a volumetric productivity of 6.2 g l–1 h–1. The optimum ratio for sugar beet arabinan was α-l-arabinofuranosidase (20.4 U ml–1) and endo-1,5-α-l-arabinanase (3.1 U ml–1) of 8:1 at pH 6.0 and 75 ºC for 1.5 h. Under the optimum ratio, 16.4 g l–1 l-arabinose was obtained from 20 g l–1 sugar beet arabinan after 2 h, with a conversion yield of 82% and a volumetric productivity of 8.1 g l–1 h–1. The hydrolytic properties for arabino-oligosaccharides of α-l-arabinofuranosidase and endo-1,5-α-l-arabinanase from C. saccharolyticus demonstrated the potential in the commercial production of l-arabinose.

키워드

저자

• Yu-Ri LIM [ Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea. ]
• Soo-Jin YEOM [ Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea. ]
• Young-Su KIM [ Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea. ]
• Chang-Su PARK [ Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea. ]
• Deok-Kun OH [ Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576