Earticle

초록

영어

Several pathogenic organisms, such as Vibrio Colera, E. coli, Helicobacter pylori, and influenza virus A and B, recognize a sialic-acid-containing carbohydrate receptor structure on target cells. Therefore, sialyl compounds may function as soluble receptor analog to attachment of the pathogens on the cell surface receptors. In this study, Biosynthesis of sialyllactose was investigated using calcium alginate blends with gelatin beads. The trypsin entrapment using Ca2+-alginate and gelatin mixture was experimentally optimized under various conditions. The substrate, N-α-benzoyl-DL-arginine-4-nitroanilide (BAPNA), was used for the confirmation of the trypsin activity. The biosynthesis for sialyllactose using several glycosyltransferases of the pathway has been built up. Five enzymes involving the biosynthesis were over-expressed in E.coli BL21 (K-12) and entrapped by Ca2+-alginate and gelatin mixture. The entrapment of five enzymes may guarantee the high stabilities of the enzymes in biosynthesis

키워드

저자

• Il-Hyang PARK [ Interdisciplinary Program of Bioengineering, Seoul National University, Seoul, 151-744. ]
• Jae-Hun LEE [ Interdisciplinary Program of Bioengineering, Seoul National University, Seoul, 151-744. ]
• Yun-Hee CHOI [ Interdisciplinary Program of Bioengineering, Seoul National University, Seoul, 151-744. ]
• Byung-Gee KIM [ School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-744. ]
• Tak-Jin KANG [ Chemical and Biological Engineering, Dongguk University, Seoul. ]

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576