Earticle

초록

영어

Galactooligosacchride (GOS) as a prebiotic is known to be beneficial to the growth of human intestinal microbial flora, and thereby led eventually to control the human diseases. Typically GOS is synthesized by β-galactosidase (β-gal)-catalyzed enzymatic reaction on lactose. During this reaction (transgalactosylation), galactose moiety is known to be transferred to hydroxyl group of lactose, and thereby GOS is synthesized.
There have been lots of reports related to GOS production by β-galactosidase and immobilized β -galactosidase. Recently, in order to increase the productivity of GOS, various reactor systems and operational strategies have been studied. In our previous studies, it was demonstrated that active Escherichia coli (E.coli) β-gal inclusion bodies (IBs) were produced by the addition of a repressor (α-methyl D-glucospyranoside) or an inducer analog (D-fucose) after induction of the araBAD promoter system in E.coli. Inaddition, we have operated successfully a packed-bed reactor for hydrolysis of o-nitrophenyl-ß-D-galactoside using immobilized β-gal IBs-containing E.coli cells. Previously, it has been reported that enzyme IBs in E.coli expression system were expressed as biologically active IBs. However, there have been little numbers of report that have explored an enzyme reactor using active IBs. The aim of this study is to investigate whether β-gal IBs-containing E.coli cell can synthesize GOS by transgalactosylation reaction, and, in addition, to explore the optimal condition of GOS synthesis by this β-gal IBs-containing E.coli cell. Interestingly, this study is the first trial for GOS synthesis by β-gal IBs. Because, if we can use active β-IBs as biocalayst, β-IB utilization will be more convenient and economic in enzymatic GOS synthesis, active β-gal IBs might be applied to the enzymatic transgalactosylation reaction without any purification steps. That is, a whole E.coli cells containing active β-gal IBs will be able to be directly used for GOS synthesis.

키워드

저자

• Ji-Hyeon YEON [ Dept of Biotechnology, Chungju National University, Jeungpyung, Chungbuk 368-701. ]
• Hye-Ji KIM [ Dept of Biotechnology, Chungju National University, Jeungpyung, Chungbuk 368-701. ]
• Hyeon-Beom SEO [ Dept of Biotechnology, Chungju National University, Jeungpyung, Chungbuk 368-701. ]
• Kyung-Hwan JUNG [ Dept of Biotechnology, Chungju National University, Jeungpyung, Chungbuk 368-701. ]

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발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576