Earticle

초록

영어

Highly stable and active nanoscale enzyme reactor (NER) [1] has been developed by adsorbing and crosslinking glucose oxidase (GOx) in polyaniline nanofibers (PANFs). PANFs, prepared by oxidative polymerization with ammonium persulfate as an oxidant [2], contains nanometer-scale pores that enable the NER approach. The simple addition of enzyme crosslinking step resulted in highly stable and active enzyme systems. For example, the activities of adsorbed GOx (ADS) and NER were 0.040 and 0.382 (A500/min) per 0.1 mg PANFs, respectively. This 9.6-fold increase of enzyme activity can be explained by the improved enzyme loading of NER because the enzyme crosslinking can effectively prevent the enzyme leaching from PANFs. Stability-wise, ADS retained only 50% of initial activity after 5 day incubation at room temperature while NER maintained more than 90% of initial activity in the same condition for two months. To demonstrate the feasible application of highly stable and active NERs in conductive PANFs, the operation of biofuel cells (BFCs) were tested with enzyme anodes based on ADS and NER. The detailed result will be given in the presentation.

키워드

저자

• Hyeongseok KIM [ Department of Chemical and Biological Engineering, Korea University, Seoul 136-701. ]
• Inseon LEE [ Department of Chemical and Biological Engineering, Korea University, Seoul 136-701. ]
• Yongchai KWON [ Department of Chemical and Environmental Technology, Inha Technical College, Incheon 402-752. ]
• Jung-Heon LEE [ Department of Chemical Engineering, Chosun University, Gwangju 501-759. ]
• Jungbae KIM [ Department of Chemical and Biological Engineering, Korea University, Seoul 136-701. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576